2. Structure elucidation

Our expertise comprises structure elucidation of AGPs and other plant polysaccharides by different methods:

  • Determination of molecular weight by GPC und LLS-detection
  • Structure elucidation of polysaccharides by e.g. partial hydrolyses, acetylation and methylation analyses, GLC with FID and MS detection, NMR)
  • Determination of amino acid composition of AGPs
  • Photometric quantification of  hydroxyproline
  • Elucidation of structure-activity relationships in combination with immunomoduling activities (see below)

 

wattle blossom model

Simplified structural model of an AGP

 

 

 

glycan model

Structural proposal for the arabinogalactan part of the AGP from aerial parts of E. purpurea

 

Selected publications:

Pfeifer L, Shafee T, Johnson KL, Bacic A, Classen B:
Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.
Scientific Reports (2020) 10: 8232.link

Happ K, Classen B:
Arabinogalactan-proteins from the liverwort Marchantia polymorpha L., a member of a basal land plant lineage, are structurally different to those of angiosperms.
Plants (2019) 8 (11): 460.link

Bartels D, Classen B:
Structural investigations on arabinogalactan-proteins from a lycophyte and different monilophytes (ferns) in the evolutionary context.                                                              Carbohydrate Polymers (2017) 172: 342-351. link     

Bartels D, Baumann A, Maeder M, Geske T, Heise EM, v Schwartzenberg K, Classen B:
Evolution of plant cell wall: Arabinogalactan-proteins from three moss genera show structural differences compared to seed plants.
Carbohydrate Polymers (2017) 163: 227–235. link

Duchow S, Dahlke RI, Geske T, Blaschek W, Classen B:
Arabinogalactan-proteins stimulate somatic embryogenesis and plant propagation of Pelargonium sidoides.
Carbohydrate Polymers (2016) 152: 149-155. link

Göllner EM , Blaschek W, Classen B:
Characterization of a Yariv precipitated arabinogalactan-protein from fruits of rye (Secale cereale L.).
Pharmazie (2013), 68: 631-635. link

Göllner EM, Utermöhlen J, Kramer R and Classen B:
Structure of arabinogalactan from Larix laricina and its reactivity with antibodies directed against type-II-arabinogalactans
Carbohydrate Polymers (2011) 86(4): 1739-1744 link

 Göllner EM, Ichinose H, Kaneko S, Blaschek W and Classen B:
An arabinogalactan-protein from whole grain of Avena sativa L. belongs to the wattle-blossom type of arabinogalactan-proteins
J Cereal Science (2011), 53: 244-249 link