2. Structure elucidation

Our expertise comprises structure elucidation of AGPs and other plant polysaccharides by different methods:

  • Determination of molecular weight by GPC und LLS-detection
  • Structure elucidation of polysaccharides by e.g. partial hydrolyses, acetylation and methylation analyses, GLC with FID and MS detection, NMR)
  • Determination of amino acid composition of AGPs
  • Photometric quantification of  hydroxyproline
  • Elucidation of structure-activity relationships in combination with immunomoduling activities (see below)

 

wattle blossom model

Simplified structural model of an AGP

 

 

 

glycan model

Structural proposal for the arabinogalactan part of the AGP from aerial parts of E. purpurea

 

Selected publications:

Göllner EM, Ichinose H, Kaneko S, Blaschek W and Classen B: An arabinogalactan-protein from whole grain of Avena sativa L. belongs to the wattle-blossom type of arabinogalactan-proteins. J Cereal Science (2011), 53: 244-249

Göllner EM, Utermöhlen J, Kramer R and Classen B: Structure of arabinogalactan from Larix laricina and its reactivity with antibodies directed against type-II-arabinogalactans. Carbohydr Pol (2011) 86(4): 1739-1744.

Göllner EM, Blaschek W, Classen B: Structural investigations on arabinogalactan-protein from wheat, isolated with Yariv reagent. J Agr Food Chem (2010) 58(6): 3621-3626.

Classen B: Characterization of an arabinogalactan-protein from suspension culture of Echinacea purpurea. Plant Cell Tiss Org Cult (2007) 88: 267-275.

Thude S, Classen B: High molecular weight constituents from roots of Echinacea pallida: An arabinogalactan-protein and an arabinan. Phytochemistry (2005) 66: 1026-1032.

Classen B, Mau SL, Bacic A: The arabinogalactan-proteins from pressed juice of Echinacea purpurea belong to the “hybrid” class of hydroxyproline-rich glycoproteins. Planta Med (2005) 71:59-66.

Classen B, Witthohn K, Blaschek W: Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the ß-glucosyl Yariv reagent. Carbohydr Res (2000) 327: 497-504.