1. Isolation of AGPs

Echinacea purpurea L. Moench      callus culture of  Echinacea purpurea

Echinacea purpurea L. Moench © Andreas Bossy     Callus culture of E. purpurea

 

As basic material we use different medicinal plants, which are used in rational phytotherapy as unspecific immunstimulants (e.g. Echinacea sp., Baptisia tinctoria, Viscum album). Furthermore we isolate AGPs from different cereals (Avena sativa, Secale cereale, Triticum aestivum) and from plant cell cultures. Suspension cultures offer the possibility to produce AGPs independent from environmental impact in huge amounts under constant conditions. Isolation of AGPs from suspension cultures is easy, because they are released into the culture medium. Recently we concentrate also on search of AGPs in primitive land plants (mosses, lycopods, ferns), as well as in algae and marine angiosperms.

An isolation of pure AGPs is possible by a very specific precipitation step. The so-called Yariv´s reagent, which is synthesized in our group, reacts specifically with AGPs and allows an easy and effective purification of these macromolecules.

 

 

Yariv reagent

Structure of the β-glucosyl Yariv reagent (Glc = β-Glc)